10x Library

Support Documentation

Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation

Cell Type Annotation Strategies for Single Cell ATAC-Seq Data

Visium Spatial Gene Expression Imaging Guidelines

Sequencing Guidelines for Single Cell Copy Number Variant Detection

Quality Control of Cell Surface Protein Labeling using Flow Cytometry

Interpreting Cell Ranger ATAC Web Summary Files for Single Cell ATAC Assay

Chromium Single Cell 3ʹ v3 Reagent, Workflow & Software Updates

Chromium Next GEM Single Cell V(D)J v1.1: Reagent & Workflow Updates

Chromium Next GEM Single Cell 3ʹ v3.1: Reagent & Workflow Updates

Chromium Next GEM Single Cell ATAC v1.1 Reagent, Workflow and Software Updates

Guide RNA Specifications Compatible with Feature Barcoding technology for CRISPR Screening

Sequencing Metrics & Base Composition of Chromium Single Cell ATAC Libraries

Biological & Technical Variation in Single Cell Gene Expression Experiments

Sequencing Metrics & Base Composition of Sequencing Reads of Chromium Single Cell DNA Libraries

Assay Scheme & Configuration of Chromium Single Cell DNA Libraries

Resolving Cell Types as a Function of Read Depth and Cell Number

Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® NovaSeq®

Chromium Genome v2 Libraries – Sequencing Metrics for Illumina® Sequencers

Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® Sequencers

Comparison between Bioanalyzer and Fragment Analyzer Traces for QC of Chromium Genome v2 Libraries

Sample Preparation Recommendations for the Chromium Genome Kit

Comparison between Bioanalyzer and TapeStation traces for QC of Chromium Genome Libraries

Target Enrichment Using Custom Baits With The Chromium Genome Reagent Kit

Instructions to Download and Process FASTQs of 1.3M Brain Cells

Base Composition of Sequencing Reads of Chromium Single Cell 3’ v2 Libraries

Instructions to Download and Process BAM files of 1.3 Million Brain Cells

Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions

Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries

Chromium Genome v2 Libraries – Gel Bead Oligo Primer Contamination Affects Sequencing & Application Performance

Assay Scheme and Configuration of Chromium Single Cell 3′ v2 Libraries

Chromium Genome Libraries – Sequencing Metrics for Illumina® NovaSeq®

Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® NovaSeq®

Introduction to Linked-Read Technology for a More Comprehensive Genome & Exome Analysis

Single-stranded DNA Damage and its Effects on Chromium Genome Application Performance

SPRIselect:DNA Ratios Affect the Size Range of Library Fragments

QC of Chromium Genome v2 Libraries: Qualitative Evaluation Using Agilent Bioanalyzer

Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications

QC of Chromium Genome Libraries: Qualitative Evaluation Using Agilent Bioanalyzer

QC of Chromium Genome Libraries: Quantitative Evaluation Using qPCR

10x Barcode Continuity during Library Sequencing

Chromium Single Cell Applications – Guidelines for Optimal Sample Preparation

Importance of Cell Stock Concentration for Accurate Target Cell Recovery

Chromium Genome Application Performance as a Result of DNA Quality & Sequencing Depth

Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® Sequencers

Assay Scheme and Configuration of Chromium Genome v2 Libraries

Demonstrated Protocols

Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing

Visium Spatial Protocols – Tissue Preparation Guide

Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols

Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing

Single Cell V(D)J Demonstrated Protocol Compatibility Table

Single Cell Gene Expression Demonstrated Protocol Compatibility Table

Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols

Nuclei Isolation for Single Cell ATAC Sequencing

Isolation of Nuclei for Single Cell DNA Sequencing

Tumor Dissociation for Single Cell RNA Sequencing

Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing

Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing

Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing

HMW DNA Extraction from Fresh Frozen Tissue

Chromium Exome Protocol Time Planner (v2 Chemistry)

Moss Protoplast Suspension for Single Cell RNA Sequencing

Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing

HMW DNA QC

Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing

DNA Size Selection

Methanol Fixation of Cells for Single Cell RNA Sequencing

Isolation of Nuclei for Single Cell RNA Sequencing

Chromium Exome Demonstrated Protocol (v2 Chemistry)

Single Cell Protocols – Cell Preparation Guide

Salting Out Method for DNA Extraction from Cells

Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling

DNA Extraction from Single Insects

HMW DNA Extraction from Whole Blood

Visium Spatial Gene Expression Protocol Planner

Chromium Connect Quick Reference Cards

Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)

Chromium Connect User Guide

Visium Spatial Gene Expression Reagent Kits User Guide

Visium Spatial Tissue Optimization Reagents Kits User Guide

Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening

Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry)

Chromium Controller Readiness Test with Chromium Next GEM Test Chip

Chromium Next GEM Training Kit User Guide

Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry)

Selecting the Correct Single Cell Gene Expression User Guide

Selecting the Correct Single Cell Immune Profiling User Guide

Chromium Single Cell V(D)J Reagent Kits User Guide (v1.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein

Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein

Selecting the Correct Single Cell ATAC User Guide

Chromium Single Cell V(D)J Reagent Kits (v1.1 Chemistry)

Chromium Genome Reagent Kit (v1 Chemistry)

Chromium Single Cell V(D)J Reagent Kits User Guide (v1 Chemistry) with Feature Barcoding technology for Cell Surface Protein

Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for CRISPR Screening

Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry)

Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for Cell Surface Protein

Chromium Single Cell ATAC Reagent Kits User Guide (v1 Chemistry)

Chromium Single Cell DNA Reagent Kits User Guide

Chromium Single Cell DNA Training Kit User Guide

Chromium Controller Readiness Test

Chromium Single Cell Controller Readiness Test

Chromium Genome Protocol Time Planner (v2 Chemistry)

Chromium Genome Reagent Kit, Quick Reference Cards (v2 Chemistry)

Chromium Genome Reagent HT Kit, Quick Reference Cards (v2 Chemistry)

Chromium Single Cell 3′ v2 Reagent Kit, Quick Reference Cards (v2 Chemistry)

Chromium Single Cell 3′ Protocol Time Planner (v2 Chemistry)

Chromium Genome Reagent Kit (v2 Chemistry)

Chromium Single Cell 3′ Reagent Kits User Guide (v2 Chemistry)

Chromium Single Cell V(D)J Reagent Kits (v1 Chemistry)

Chromium Controller Training Kit