Everyday de novo Assembly


Chromium de novo Assembly Solution

True Diploid Assembly with Supernova

Discover the true genome with Supernova, our de novo assembly software, and open the door to low-cost,
everyday diploid assemblies. Unlock sample-specific sequences, probe diploid genome structure,
and remove the need for a reference sequence of any kind.

Our Solution Features

  • True diploid assembly unlike other methods
  • Fast and simple de novo assembly workflow
  • Go from sample to assembly in <2 weeks
  • 10 Mb scaffold size and long range accuracy
  • High-quality assembly from as little as 1 ng of DNA
  • Validated and supported for human genomes*
View the Workflow
Genome Solution Suite

*Note on non-human samples: Supernova is a de novo assembly program that has been designed to assemble germline human genomes, from data generated using Chromium Genome Reagent Kits. 10x Genomics has validated Chromium Genome Reagent Kits and Supernova only on human genomes. Therefore, using Supernova to assemble non-human genomes is experimental and may have unexpected features that limit assembly quality. Despite this, many users have successfully assembled non-human genomes using Supernova.

Assembly Controller

Chromium Controller

Compact, Sleek, Efficient.

The compact, sleek Chromium Controller rapidly and efficiently combines large partition numbers with a massively diverse barcode library to generate >100,000 barcode-containing partitions in a matter of minutes. The Chromium Controller fits on a standard laboratory bench and allows a user to run any Chromium Solution, from genome to single cell analysis.

View Our Controller Gallery

Product List

Chromium de novo Assembly Solution

Available for use only with the Chromium Controller instrument Part Number 120223 and 120246, or the Chromium Single Cell Controller instrument Part Number 120263 and 120212.

Reagents & Consumables

Product Code
Chromium Genome Chip Kit v2
48 rxns
Chromium Genome HT Library & Gel Bead Kit v2
96 rxns
Chromium Genome Library Kit & Gel Bead Kit v2
16 rxns
Chromium i7 Multiplex Kit
96 rxns


The Supernova Assembler
Assembly Datasets
Scientific Seminars

Dissecting Complex Systems with Multidimensional Data

  • Sarah Garcia, 10x Genomics, Inc.
March 21, 2019

Genome Assembly of the Mind-controlling Fly Pathogen E. Muscae Via 10x Genomics

  • Carolyn Elya, University of California, Berkeley
March 21, 2017
User Group Meeting – San Francisco, CA

de Novo Assembly of a Large Size Genome: the Olive Fruit Fly

  • Jiannis Ragoussis, McGill University
February 14, 2017
AGBT Annual Meeting – Hollywood, FL
Poster Hall

Assembly of individual chromosomes at megabase scale using 10x Linked-Reads

  • Genome Informatics
    Cold Spring Harbor Laboratory, New York
    November 1 – November 4, 2017

Order your 10x system, today.