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技术说明
实验方案演示
用户指南
Genome Sequencing
Single Cell Gene Expression
Single Cell Immune Profiling
Single Cell CNV
Single Cell ATAC
Spatial Gene Expression Profiling
Chromium Connect
Exome Sequencing
Targeted Gene Expression
de novo
Assembly
Single Cell Gene Expression and CRISPR Screening
Data Analysis
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技术说明
Interpreting Intronic and Antisense Reads in 10x Genomics Single Cell Gene Expression Data
Chromium Next GEM Single Cell 3ʹ v3.1: Cell Multiplexing
Cell Type Annotation Strategies for Single Cell ATAC-Seq Data
Sequencing Metrics & Base Composition of Single Cell Multiome ATAC Libraries
Chromium Next GEM Single Cell 5′ v2 (Dual Index): Reagent, Workflow & Performance Updates
Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation
Targeted Chromium Single Cell Gene Expression: Pre-designed Panel Performance Metrics
Chromium Next GEM Single Cell 3ʹ v3.1: Dual Index Libraries
Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries
Interpreting Cell Ranger ARC Web Summary Files for Single Cell Multiome ATAC + Gene Expression Assay
Targeted Gene Expression Custom Panel Performance Metrics and Best Practices
Sequencing Metrics & Base Composition of Single Cell 5′ v2 Dual Index Libraries
Sequencing Metrics & Base Composition of Single Cell 3′ v3.1 Dual Index Libraries
Chromium Connect: Consistent Automated Single Cell 5′ Gene Expression and V(D)J Library Generation
Interpreting Cell Ranger Web Summary Files for Single Cell Gene Expression Assays
Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Data Comparison
Targeted Visium Spatial Gene Expression: Pre-designed Panel Performance Metrics
Visium Spatial Gene Expression Imaging Guidelines
Sequencing Guidelines for Single Cell Copy Number Variant Detection
Quality Control of Cell Surface Protein Labeling using Flow Cytometry
Interpreting Cell Ranger ATAC Web Summary Files for Single Cell ATAC Assay
Chromium Single Cell 3ʹ v3 Reagent, Workflow & Software Updates
Chromium Next GEM Single Cell V(D)J v1.1: Reagent & Workflow Updates
Chromium Next GEM Single Cell 3ʹ v3.1: Reagent & Workflow Updates
Chromium Next GEM Single Cell ATAC v1.1 Reagent, Workflow and Software Updates
Guide RNA Specifications Compatible with Feature Barcoding technology for CRISPR Screening
Sequencing Metrics & Base Composition of Chromium Single Cell ATAC Libraries
Biological & Technical Variation in Single Cell Gene Expression Experiments
Sequencing Metrics & Base Composition of Sequencing Reads of Chromium Single Cell DNA Libraries
Assay Scheme & Configuration of Chromium Single Cell DNA Libraries
Resolving Cell Types as a Function of Read Depth and Cell Number
Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® NovaSeq®
Chromium Genome v2 Libraries – Sequencing Metrics for Illumina® Sequencers
Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® Sequencers
Comparison between Bioanalyzer and Fragment Analyzer Traces for QC of Chromium Genome v2 Libraries
Sample Preparation Recommendations for the Chromium Genome Kit
Comparison between Bioanalyzer and TapeStation traces for QC of Chromium Genome Libraries
Target Enrichment Using Custom Baits With The Chromium Genome Reagent Kit
Instructions to Download and Process FASTQs of 1.3M Brain Cells
Base Composition of Sequencing Reads of Chromium Single Cell 3’ v2 Libraries
Instructions to Download and Process BAM files of 1.3 Million Brain Cells
Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions
Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries
Chromium Genome v2 Libraries – Gel Bead Oligo Primer Contamination Affects Sequencing & Application Performance
Assay Scheme and Configuration of Chromium Single Cell 3′ v2 Libraries
Chromium Genome Libraries – Sequencing Metrics for Illumina® NovaSeq®
Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® NovaSeq®
Introduction to Linked-Read Technology for a More Comprehensive Genome & Exome Analysis
Single-stranded DNA Damage and its Effects on Chromium Genome Application Performance
SPRIselect:DNA Ratios Affect the Size Range of Library Fragments
QC of Chromium Genome v2 Libraries: Qualitative Evaluation Using Agilent Bioanalyzer
Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications
QC of Chromium Genome Libraries: Qualitative Evaluation Using Agilent Bioanalyzer
QC of Chromium Genome Libraries: Quantitative Evaluation Using qPCR
10x Barcode Continuity during Library Sequencing
Chromium Single Cell Applications – Guidelines for Optimal Sample Preparation
Importance of Cell Stock Concentration for Accurate Target Cell Recovery
Chromium Genome Application Performance as a Result of DNA Quality & Sequencing Depth
Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® Sequencers
Assay Scheme and Configuration of Chromium Genome v2 Libraries
实验方案演示
Nuclei Isolation from Adult Mouse Brain Tissue for Single Cell RNA Sequencing
Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing
SPRIselect Library Concentration for Targeted Gene Expression
Methanol Fixation, H&E Staining & Imaging for Visium Spatial Protocols
Visium Spatial Slide Reset
Nuclei Isolation from Complex Tissues for Single Cell Multiome ATAC + Gene Expression Sequencing
Nuclei Isolation from Embryonic Mouse Brain Tissue for Single Cell Multiome ATAC + Gene Expression Sequencing
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
Methanol Fixation, Immunofluorescence Staining & Imaging for Visium Spatial Protocols
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
Visium Spatial Protocols – Tissue Preparation Guide
Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols
Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing
Single Cell V(D)J Demonstrated Protocol Compatibility Table
Single Cell Gene Expression Demonstrated Protocol Compatibility Table
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Nuclei Isolation for Single Cell ATAC Sequencing
Isolation of Nuclei for Single Cell DNA Sequencing
Tumor Dissociation for Single Cell RNA Sequencing
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
HMW DNA Extraction from Fresh Frozen Tissue
Chromium Exome Protocol Time Planner (v2 Chemistry)
Moss Protoplast Suspension for Single Cell RNA Sequencing
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
HMW DNA QC
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
DNA Size Selection
Methanol Fixation of Cells for Single Cell RNA Sequencing
Isolation of Nuclei for Single Cell RNA Sequencing
Chromium Exome Demonstrated Protocol (v2 Chemistry)
Single Cell Protocols – Cell Preparation Guide
Salting Out Method for DNA Extraction from Cells
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling
DNA Extraction from Single Insects
HMW DNA Extraction from Whole Blood
用户指南
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing
Chromium Connect Quick Reference Cards
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Multiplexing
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening
Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)
Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry)
Targeted Gene Expression – Spatial User Guide
Chromium Single Cell 5′ Reagent Kits User Guide (v2 Chemistry Dual Index)
Chromium Single Cell 5′ Reagent Kits User Guide (v2 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Immune Receptor Mapping
Chromium Connect User Guide
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening and Cell Multiplexing
Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide
Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for CRISPR Screening
Targeted Gene Expression – Single Cell User Guide
Visium Spatial Gene Expression Protocol Planner
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry Dual Index)
Visium Spatial Gene Expression Reagent Kits User Guide
Visium Spatial Tissue Optimization Reagents Kits User Guide
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry)
Chromium Controller Readiness Test with Chromium Next GEM Test Chip
Chromium Next GEM Training Kit User Guide
Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry)
Selecting the Correct Single Cell Gene Expression User Guide
Selecting the Correct Single Cell Immune Profiling User Guide
Chromium Single Cell V(D)J Reagent Kits User Guide (v1.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Selecting the Correct Single Cell ATAC User Guide
Chromium Single Cell V(D)J Reagent Kits (v1.1 Chemistry)
Chromium Genome Reagent Kit (v1 Chemistry)
Chromium Single Cell V(D)J Reagent Kits User Guide (v1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell ATAC Reagent Kits User Guide (v1 Chemistry)
Chromium Single Cell DNA Reagent Kits User Guide
Chromium Single Cell DNA Training Kit User Guide
Chromium Controller Readiness Test
Chromium Single Cell Controller Readiness Test
Chromium Genome Protocol Time Planner (v2 Chemistry)
Chromium Genome Reagent Kit, Quick Reference Cards (v2 Chemistry)
Chromium Genome Reagent HT Kit, Quick Reference Cards (v2 Chemistry)
Chromium Single Cell 3′ v2 Reagent Kit, Quick Reference Cards (v2 Chemistry)
Chromium Single Cell 3′ Protocol Time Planner (v2 Chemistry)
Chromium Genome Reagent Kit (v2 Chemistry)
Chromium Single Cell 3′ Reagent Kits User Guide (v2 Chemistry)
Chromium Single Cell V(D)J Reagent Kits (v1 Chemistry)
Chromium Controller Training Kit